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The suit also alleges that Alto Gary, the Texans’ cheerleader coach, “made threats of imminent bodily harm” to the former cheerleaders. Allred would not elaborate on that allegation and said the former cheerleaders were focused on settling the issue through civil court actions and not criminal complaints.

Former cheerleader Kelly Neuner said the Texans “did not care about my rights as a professional as a woman and as a part of the team. … This organization does so much good work in the Houston community and throughout Texas. However, they should treat their cheerleaders with the same respect and dignity.”

Allred said the Texans and their fans owe current and former cheerleaders respect and proper pay for their performance.

“Pay these women what they deserve, and be sure you pay them for every minute they work,” Allred said. “Everyone who supports this team while how these cheerleaders are being treated are complicit.”

The lawsuit Friday contains many of the same elements as a May 21 case filed on behalf of a former cheerleader who is seeking class action status on behalf of other former Texans cheerleaders.

Allred said the five women she represents would not seek class action status because each has a different claim against the team.

Some contracts require that legal disputes be submitted to arbitration, but Spurlock said there are discrepancies in that regard among different versions of cheerleader contracts and that attorneys hope to proceed through the federal court system.

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Italy

Welcome to Cortina d’Ampezzo

The Italian supermodel of ski resorts, Cortina d'Ampezzo is icy, pricey and undeniably beautiful. The town's stone church spires and pleasant cascading piazzas are framed by magnificent alps. It doubles as a slightly less glamorous but still stunning summertime base for hiking, biking and rock climbing.

We've pre-picked the best hotels, hostels and bed and breakfasts to make your stay as enjoyable as possible.

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Skiing in Italy: find your perfect resort

Italy’s northern borders are delineated by an arc of majestic mountain peaks, including some of the tallest peaks in the Alps in the west and the spectacularly beautiful Dolomites i...

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Starting at $36.39

Ready to go? Get to the heart of Cortina d’Ampezzo with one of Lonely Planet's in-depth, award-winning guidebooks.

Dolomiti and First World War Sites Ski Tour from Cortina d'Ampezzo

The centre point of the First World War ski tour is the symbolic mountain of the 1st world war, Mount Col di Lana. The 80 km (49 mi) tour takes you around this mountain. Everywhere, you will find traces of World War 1: artefacts from the war era, trenches running right through the rocks, parapet walks and forts. At the Cinque Torri the Italian trenches can be seen and on the Lagazuoi, you can visit the Austrian machine gun post and just below it the guard post with the officers’ shack, with skis at its feet. The Armentarola slope is one of the most fascinating environments of the Dolomites. At the bottom, a horse-drawn tow takes the skiers to the slopes of the Badia Valley, where they proceed to Arabba, Padon and Marmolada. The stop for lunch is in a traditional mountain refuge to taste some traditional dishes.

Kronplatz Full-Day Guided Ski or Snowboard Tour with Transport

Aday on the slopes in Kronplatzreally does offer everything you could wish for on holiday. This mighty mountain between Bruneck-Brunico, St. Vigil-San Viglio and Valdaora-Olang has32 state-of-the-art liftsserving119 km of pistescatering for all abilities:beginners,cruisersandexperts. Intermediate skiers will appreciate the wide runs down into the valley, while ambitious skiers and snowboarders can test their mettle on theBlack Five.The tour starts with a departure from the hotel at 9.00 a.m. You will be in Kronplatz around 10.00 a.m. and after a short break for a coffee, you will start the day on the snow. According to your ski or snowboard level, and your requests, the guide will choose the best slopes. The stop for lunch is in a traditional mountain refuge to taste some traditional dishes. Youwill be back at the hotel around 4.00 p.m. Technical details: over 100km of slopes 30 refuge skipass: Dolomiti Superski 5 black slopes

Hidden Valley Ski Tour

The Hidden Valley drops-in from the top of the Lagazuoi mountain at 2,752m, adjacent to the famous Rifugio. The Fanes mountain range can be seen to the right of the run while descending and is just a majestic sight. The valley walls to the left are smaller but scattered with frozen waterfalls, one of which you ski right under. It isn’t a water-fall, really, this is water that has seeped through the rock to be frozen on its passage to the valley floor. The colours, shapes and scale are mesmerising. The run twists and turns for 7 km, creating a mysterious and secret ambience. Once at the bottom of the trail, you have the option of taking the horse drag, a great experience, where you hold on to ropes pulled by a pair of horses as they take you along the flat frozen river bed to the next lift. Technical details: refuges skipass: Dolomiti Superski

Dolomiti Ski Tour: Sellaronda from Cortina d'Ampezzo

We will reach Corvara with a boarderline’s shuttle. From Corvara we are able to enter Sella Ronda directly. We go up to Gardena and take the Dantercepies run down to Wolkenstein in Gardena Valley. In the middle of the village we enter the gondola up to the Ciampinoi peak. A small side-trip brings us on the Col Rodella. Here we have a view down to the valley of Fassa, we then go down-hill to the Lupo Bianco hut, a nice place to take a break.The gondola gets you in the main ski area of Canazei. Then you're off on an 8km (4.5 mi) long run, to Arabba. Soon it gets really steep; beginners can take a less steep passing route that runs beside this. Then a lift brings will bring you back to Corvara and Colfosco and after a short and wide run and with the next chairlift you reach a bear hut. From here there is a great view and in the background, you can see the Marmolada.Then the tour goes on with the next run to the lift station short behind the Campologno pass. A chairlift takes you up to the Crep de Munt on the direct run to Corvara.

Dolomiti Ski Tour: Super 8 Lagazuoi and 5 Torri from Cortina d'Ampezzo

The Ski Tour winds its way around the mountains in an 8-shape, along beautiful and varied slopes, and can be accessed via the lifts of Bai de Dones and Passo Giau, to Passo Falzarego and Armentarola. FromLagazuoi, you'll descend into the lovely Hidden Valley through magnificent mountain scenery and finishing with a horse-drawn lift ride. High altitude refuges with panoramic terraces, frozen waterfalls, and high quality services all contribute to a unique experience which even includes a horse powered ski pull. The Super8 ski tour is a must-do experience when skiing in Cortina, offering breathtaking views of the most famous Dolomite peaks like the Tofana, Pelmo, Civetta, Marmolada, Fanes, Averau, 5 Torri, and Conturines.Before you begin the tour you have to stop in order to admire the view at the top of Lagazuoi (2800 mt.) from the Tofana, up to the Marmolada and Sella group.

Ski Tour Cortina d'Ampezzo: Faloria-Cristallo

The departure is at 9am or at 1pm.We will take Faloria cable car and ski in this area. The skiing here is hidden from Cortina, there are slopes of all levels of difficulty, all with a marvelous view over the Ampezzo Valley.There are many mountain refuges in this area to enjoy a little break, if needed. Then we move to Cristallo and discover this area.Faloria2 cable cars, 5 chair lifts, 1 ski lift cable car Cortina-Mandres-Faloria, vertical drop of 893 meters. Chair lift Girilanda-Tondi 1 and 2, vertical drop of 325 meters. Ski lift of Tondi, vertical drop of 134 meters. Chair lift A.A. Alpe Faloria-Tondi "Vitelli", vertical drop of 340 meters. Chair lift Rio Gere-Pian de Ra Bigontina, vertical drop of 67 meters. Chair lift A.A. Pian Bigontina-Costa Faloria, vertical drop of 313 meters.Cristallo2 Chair lifts chair lift Rio Gere-Son Forca, vertical drop of 532 meters. Chair lift Padeon-Son Forca, vertical drop of 208 meters.

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The resolution of GRAD analysis would be affected by the sequencing depth and the fetal DNA fraction in the maternal plasma sample. We performed computer simulation analyses to determine the number of SNPs required for achieving one classification of maternal inheritance with an overall accuracy of over 95% ( Clearance Outlet Locations With Credit Card Cheap Online Stuart Weitzman Pipeheist Ponyhair Pumps Sneakernews For Sale Cheap Sale Countdown Package uNezMoowtS
). With a sequencing depth of 250×, we would be able to achieve close to single-nucleotide resolution for the deduction of maternal inheritance when the fetal DNA fraction was above 20%. When the fetal DNA fraction was 10% and a sequencing depth was at 300×, maternal inheritance could be deduced in 1 of every 5 SNPs with an accuracy of over 95%, but the resolution of maternal inheritance would drop to 1 classification per 30 SNPs with a sequencing depth of 150×.

Fig. S3.

Simulation analysis for the number of SNPs per one accurate GRAD classification of maternal inheritance for different fetal DNA fractions and sequencing depths.

Facilitated by the high-sequencing depth of a non-PCR–amplified library of the maternal plasma DNA sample, we investigated if certain base positions in the genome would be preferentially represented at the ends of plasma DNA fragments. In this regard, we showed that plasma DNA fragments carrying the most prevalent 0.5% of plasma DNA ends represented 3.5% of all DNA fragments. Across the genome, 25% of the fragments had at least one identical fragment that shared the same ending sites at both ends. If the cleavage or breakage of plasma DNA was completely random, only 1.45% of the DNA molecules would be expected to have at least one counterpart sharing common ends (details are in Materials and Methods ). Had the sequencing been performed using a PCR-amplified library, then 14% of the fragments would have been wrongly assumed to be PCR duplicates and would have been filtered off.

We further investigated if there would be differences in the preferred ending sites for maternal and fetal DNA in the same cell-free DNA sample. To show this phenomenon, informative SNP loci where the mother was homozygous (genotype denoted as AA) and the fetus was heterozygous (genotype denoted as AB) were identified. In this illustrative example, the B allele would be fetal-specific, and the A allele would be shared by the mother and the fetus. The fetal-specific and shared reads covering one such informative SNP are shown in Fig. 3 . For comparison, the sequencing results of a DNA sample obtained from blood cells and artificially fragmented using sonication are also shown. Clusters of preferred ending positions could be observed among the plasma DNA data. For the plot of the probability of a genomic location being an end of DNA fragments (also referred to as the fragment end probability), three peaks were observed for each of two groups of fragments carrying the fetal-specific allele and the allele shared by the mother. These peaks represent the hotspots for the end positions of fetal- and maternal-derived DNA in maternal plasma, respectively. In contrast, the fragmentation pattern of the sonicated DNA seems to be random without such clusters, and the fragment end probability is similar across the region ( Fig. 3 ).

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